Föreläsningar och seminarier The ecDNA Epigenome: from Permissive Chromatin to Actionable Vulnerability - Cloned
Welcome to a seminar with Dr Guiping Wang from Stanford University.
About this talk
Gene regulation is a spatial process, shaped by the nuclear architecture and dynamic interactions of DNA, RNA, and protein machinery. In this seminar, Dr. Guiping Wang will share her work on developing and applying quantitative approaches to decipher these spatial principles in health and disease. She will briefly review her doctoral work on advancing Multiplexed Error-Robust Fluorescence In Situ Hybridization (MERFISH) for high-throughput spatial transcriptomics. The majority of the talk will focus on her postdoctoral research, applying quantitative genomics to extrachromosomal DNA (ecDNA). EcDNA is a key driver of oncogene amplification and therapeutic resistance in many aggressive cancers. Despite its clinical importance, its actionable vulnerabilities have remained elusive. Dr Wang will share the recent discovery of a key vulnerability in ecDNA-driven tumors: a heightened replication stress driven by pervasive transcription on ecDNA, a finding leveraged to develop a novel synthetic lethal strategy to overcome drug resistance.
About the speaker
Dr Guiping Wang earned her PhD in Chemistry from Harvard University and is currently a Postdoctoral Fellow at the Stanford University School of Medicine. She develops and applies cutting-edge imaging and sequencing methods to understand the fundamental principles linking molecular-level organization to cellular functions and pathological states.
Selected publications
- Scalable profiling of RNA-chromatin contacts by RNA-guided in situ tagmentation. In preparation. Wang G, Greenleaf WJ, Chang HY.
- Enhancing transcription replication conflict targets ecDNA positive cancers. Nature 2024;635:210–8. Tang J*, Weiser NE*, Wang G*, Chowdhry S, Curtis EJ, Zhao Y, Wong ITL, Marinov GK, Li R, Hanoian P, Tse E, Mojica SG, Hansen R, Plum J, Steffy A, Milutinovic S, Meyer ST, Luebeck J, Wang Y, Zhang S, Altemose N, Curtis C, Greenleaf WJ, Bafna V, Benkovic SJ, Pinkerton AB, Kasibhatia S, Hassig CA#, Mischel PS#, Chang HY#. *Co-first authors # co-corresponding authors
- Inducible lncRNA transgenic mice reveal continual role of HOTAIR in promoting breast cancer metastasis. eLife 2022 Dec 29;11:e79126. Ma Q*#, Yang L*, Tolentino K*, Wang G, Zhao Y, Litzenburger UM, Shi Q, Zhu L, Yang C, Jiao H, Zhang F, Li R, Tsai MC, Chen JA, Lai I, Zeng H, Li L#, Chang HY#.
- Spatial organization of the transcriptome in individual neurons. bioRxiv 2020:2020.12.07.414060. Wang G, Ang CE, Fan J, Wang A, Moffitt JR, Zhuang X. doi: https://doi.org/10.1101/2020.12.07.414060
- Structural plasticity of actin-spectrin membrane skeleton and functional role of actin and spectrin in axon degeneration. eLife 2019 May 1;8:e38730. Wang G*, Simon DJ*, Wu Z, Belsky DM, Heller E, O'Rourke MK, Hertz NT, Molina H, Zhong G, Tessier-Lavigne M#, Zhuang X#.
- Multiplexed imaging of high-density libraries of RNAs with MERFISH and expansion microscopy. Sci Rep. 2018 Mar 19;8(1):4847. Wang G, Moffitt JR, Zhuang X.
Host:
Department of Medical Biochemistry and Biophysics (contact: tamsinlindstrom@ki.se) and Cell and Molecular Biology (contact: christian.goeritz@ki.se)